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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Swainsonine Triggers Paraptosis via ER Stress and MAPK Signaling Pathway in Rat Primary Renal Tubular Epithelial Cells
doi: 10.3389/fphar.2021.715285
Figure Lengend Snippet: Effects of MAPK pathway inhibitors on SW-induced cytoplasmic vacuolization. RTECs were pretreated with SP600125 or U0126 at the indicated concentrations for 4 h and further treated with 400 μg/ml SW for 24 h (A–D) Western blotting was used to analyze the p -JNK and p -ERK protein level changes. Three independent experiments were performed. * p < 0.05, ** p < 0.01 (E,F) Inverted microscopic images of RTECs treated with SW (400 μg/ml) in the presence of SP600125 or U0126. Morphometric analysis of vacuoles was performed with three different areas and the percentage of SW-induced vacuolated cells was calculated.
Article Snippet: Bafilomycin A1 (Baf A1) (HY-100558), Rapamycin (Rapa) (HY-10219),
Techniques: Western Blot